Describe some of the modifications that polypeptide chains undergo before becoming functional proteins

Polypeptide chains undergo some modifications before they become fully functional. Some of these modifications include: proteolytic cleavage, lipidation and glycosylation. Proteolytic cleavage refers to the removal of some amino acids from a polypeptide chain by proteases in order for the protein to become active. An example of a substance that is modified through this process is insulin.