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11 September, 08:16

Why are the recombinants produced from an hfr x f - cross rarely, if ever, f+?

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  1. 11 September, 11:46
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    The F factor straightly handover the donor chromosome in the cross. H takes roughly 90 minutes to transfer the chromosome. The big share of the F factor is last to be transmitted and the conjugation tube is very subtle so the probability for a thorough transfer is low. Consequently, when the bacterial strain attempts to inoculate an Hfr plasmid into an F - strain transversely the conjugation bridge through rolling circle replication, it is infrequently completed and typically breakdowns off before the F factor section is totally transferred.
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