Explain the role of lysozyme and the freezing of the cells play in the isolation the gfp from the other bacterial proteins.

In the isolation of green fluorescent program from other bacterial protein, the lysozyme enzymatically digest the bacterial cell wall in order to deteriorate the cell by allowing the water to expand and break the cell wall. Meanwhile, the freezing of the cell will cause the bacterial cytoplasm to expand and thus rupturing the weakened cell wall.

The answer is it will allow the water to expand and break the cell wall. Lysozyme, one of the most powerful natural antibacterial and antiviral compounds known to man, has been used in foods and pharmaceuticals for over three decades as it naturally inhibits the growth.