To clone the entire coding region of p53 into the plasmid, which restriction enzyme (s) could you use to cut both your pcr product and the pcdna3 plasmid?

They protect bacterial cells from invasion by foreign DNA. Plasmid vector you want to use to clone a gene. The diagram shows the location of the recognition sites for four restrictions enzymes, BamHI. As the bacteria divide they will lose the plasmid. If a plasmid lacks a roots of replication.