Using solid-phase synthesis, you want to synthesize a 70-mer oligonucleotide with an overall yield of at least 60%. Your gene machine has a coupling efficiency of 99%. Will you be able to achieve your goal? Show the math.

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Biology

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18 April, 02:07

Using solid-phase synthesis, you want to synthesize a 70-mer oligonucleotide with an overall yield of at least 60%. Your gene machine has a coupling efficiency of 99%. Will you be able to achieve your goal? Show the math.

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Answers (1)

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Terry Moyer · Answer 1

No, The goal cannot be achieved

Explanation:

In calculating solid phase synthesis,

Yield (Y) = CE^n-1

CE is coupling efficiency

n is length of oligonucleotide

Therefore, according to the question

CE is 99% = 0.99 (99/100)

n is 70

Y = 0.99^70-1 = 0.99^69 = 0.499

Converting to percentage

0.499 x 100=49.9% approximately 50%.