Bacteria classified as GRAM+contain MANY layers.

1. Pseudomurein

2. Phospholipids

3. Cholesterol

4. Peptidoglycan

The correct answer is number 4. Peptidoblycan

Explanation:

These bacteria, when performing the Gram staining technique, have a violet or dark blue stain. Gram-positive organisms are able to retain the violet dye due to the large amount of peptidoglycan in their cell wall. Cell walls of gram-positive organisms usually lack the peripheral membrane present in gram-negative bacteria.

Peptidoglycan, also called murein, is a peptide-bound heteropolysaccharide present in the prokaryote cell wall. It is formed by two types of sugars (N-acetylmuramic acid and N-acetylglucosamine) and some amino acids.