7) Place the following list of steps into the correct sequence for creating a genetically modified organism.

a. The gene is transferred to the target organism.

b. The bacteria containing the recombinant DNA are allowed to reproduce.

c. The gene of interest is inserted into a plasmid.

d. A plasmid is isolated from a strain of bacteria.

e. The plasmid containing the recombinant DNA is inserted into bacteria.

f. The gene of interest is isolated from the cell of an organism.

Question

Biology

Babbles

7 November, 21:54

7) Place the following list of steps into the correct sequence for creating a genetically modified organism.

a. The gene is transferred to the target organism.

b. The bacteria containing the recombinant DNA are allowed to reproduce.

c. The gene of interest is inserted into a plasmid.

d. A plasmid is isolated from a strain of bacteria.

e. The plasmid containing the recombinant DNA is inserted into bacteria.

f. The gene of interest is isolated from the cell of an organism.

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Answers (1)

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Tatiana Carroll · Answer 1

F-d-b-c-e-a

Explanation:

- identification of the gene interest

- isolation of the gene of interest

- amplifying the gene to produce many copies

- associating the gene with an appropriate promoter and poly A sequence and insertion into plasmid

- multiplying the plasmid in bacteria

- transference of the construit into the recipient tissue, usually fertilized egg