At each stage of production, sterility of the preparation is a major consideration. When considering appropriate measures to implement to control microbial growth, one must think about the susceptibility of possible microbial contaminants. Apply your knowledge of the structural features of microbes and the mechanisms of action for antimicrobial agents to rank the following microbes according to their susceptibility. a. gram posb. gram negc. eveloped virusesd. endosporese. prions

least susceptible:

prions

>endospores

>gram-negative bacteria

> gram-positive bacteria

>enveloped virus

Explanation:

Prions:

Are well resistant to ultraviolet radiation that destroys the nucleic acid and inactivates viruses as they lack nucleic acid

Endospores: Could resist most agents that kills the vegetative cells most microbes are formed from.

Gram-negative bacteria: Has higher resistance to antibiotics than Gram-positive due to its outer membrane

The gram positive bacteria: Lack outer membrane and are more susceptible to antibiotics.

Enveloped viruses: Its outer lipid layer of glycoprotein and lipoproteins (envelop) are well neutralized easily