Predict the most likely effect of a two-nucleotide deletion in the middle of the intron located between exons 4 and 5 on the structure of protein A. Justify your prediction.

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The question is incomplete; however, it is important to note that the deletion of two nucleotides in an intron generally doesn't have effect on gene function, thereby they are generally neutral.

Explanation:

Introns are gene regions that were historically considered as junk DNA because they do not encode for proteins, and therefore they were thought to have no biological function. However, in the last years, it has been discovered that introns are gene regions that encode non-coding RNA (ncRNAs), which are involved in gene regulation.