A new viral pathogen has emerged, and you have been asked to develop an ELISA-based detection assay to the intact virions. In order to ensure that a mutation in a single surface protein does not render your assay obsolete, which of the following should you use? a. Fluorescence in situ hybridization (FISH) b. Molecular beaconsc. Monoclonal antibodiesd. Polyclonal antibodiese. Subunit vaccine

Question

Biology

Flowers

21 June, 05:03

A new viral pathogen has emerged, and you have been asked to develop an ELISA-based detection assay to the intact virions. In order to ensure that a mutation in a single surface protein does not render your assay obsolete, which of the following should you use? a. Fluorescence in situ hybridization (FISH) b. Molecular beaconsc. Monoclonal antibodiesd. Polyclonal antibodiese. Subunit vaccine

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Cruella · Answer 1

Fluorescence in situ hybridization (FISH)

Explanation:

Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to unknown parts of a nucleic acid sequence with a high degree of sequence complementarity. FISH can be used to detect and localize the presence or absence of specific DNA sequences on chromosomes. Can also be used to detect and localize specific RNA targets in cells.