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22 October, 16:30

How does genomic library differe from cDNA library a genomic library contains only noncoding sequences g?

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  1. 22 October, 16:35
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    The question is incomplete as it does not have the options which are:

    A) A genomic library contains coding and noncoding sequences, whereas a cDNA library contains only coding sequences.

    B) A genomic library varies, dependent on the cell type used to make it, whereas the content of a cDNA library does not.

    C) A genomic library can be made using a restriction enzyme only, whereas a cDNA library requires plasmids and DNA ligase.

    D) The genomic library can be replicated but not transcribed.

    E) The genomic library contains only the genes that can be expressed in the cell.

    Answer:

    Option-A

    Explanation:

    Two types of DNA libraries are constructed on the basis of the DNA selected for making library which could play an important role in gene cloning experiments.

    The Genomic library is constructed from the Whole-genome of the organism whereas the cDNA is constructed from the specific mRNA expressed in a cell by reverse transcriptase. Since the whole genome contains both coding and non-coding sequences whereas cDNA contains only coding sequences, therefore, is true.

    The genomic library requires restriction endonuclease along with DNA ligases whereas cDNA requires reverse transcriptase also.

    The genomic library can be replicated as well as transcribed and it is not necessary that the genomic library includes cells of a specific type.

    Thus, on the basis of this, the Option - A is the correct answer.
  2. 22 October, 19:03
    0
    1) A genomic library contains coding and noncoding sequences, whereas a cDNA library contains only coding sequences.

    2) A genomic library contains DNA fragments that express the whole genome of an organism while in cDNA library, mRNA is taken from specific cells of an organism, and then cDNA is made from that mRNA.

    3) A genomic library varies, dependent on the cell type used to make it, whereas the content of a cDNA library does not.
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