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13 February, 23:36

An experimenter has altered the 3' end of the tRNA corresponding to the amino acid methionine in such a way as to remove the 3' AC. Which of the following hypotheses describes the most likely result?

A) tRNA will not form a cloverleaf.

B) The nearby stem end will pair improperly.

C) The amino acid methionine will not bind.

D) The anticodon will not bind with the mRNA codon.

E) The aminoacylsynthetase will not be formed.

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  1. 13 February, 23:45
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    C) The amino acid methionine will not bind.

    Explanation:

    The tRNA has a nucleotide sequence that varies in length from 73 to 93 nucleotides. The 3' end of the tRNA serves as an amino acid arm. All the tRNA molecules have the trinucleotide sequence CCA at the 3' end. The base "A" is present at the 3' end. The amino acid arm serves to carry a specific amino acid according to the anticodon sequence present in its anticodon arm.

    The amino acid is esterified by its carboxyl group to the 2' - or 3'-hydroxyl group of the adenine residue that is present at the 3' end of the tRNA. Therefore, the removal of 3' AC would not allow the tRNA to bind to its corresponding amino acid (here, methionine).
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