protein purification: calculate the total yield of your protein from E. coli based on the following data. your purified protein concentration = 3mg/ml; you have a total volume of pure protein = 3.5ml, which you got it from a 250ml of e coli culture 2. western blot. what is the use of blocking your PVDF membrane after the proteins have been transferred from the gel?

Question

Biology

Malakai Watts

22 June, 07:37

protein purification: calculate the total yield of your protein from E. coli based on the following data. your purified protein concentration = 3mg/ml; you have a total volume of pure protein = 3.5ml, which you got it from a 250ml of e coli culture 2. western blot. what is the use of blocking your PVDF membrane after the proteins have been transferred from the gel?

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Joseph Clay · Answer 1

1) 0.042 mg/ml; 2) To block non-specific binding of antibodies.

Explanation:

1) Total amount of protein in the 3.5 ml: 3 mg/ml x 3.5 ml = 10.5 mg

Total yield of protein from the 250 ml of E. coli culture: 10.5 mg / 250 ml = 0.042 mg/ml

2) To block non-specific binding of antibodies.