Which of the following does not occur during Mitosis?

The correct option is 'replication of chromosomes.'

Explanation:

Mitosis can be described as a type of cell division in which two daughter cells are produced which are identical to the parent cell. crossing over or independent assortment does not occur during this process. The cells produced by this method do not have to replicate their chromosomes. Hence, this option is not true.

Other options are true like the alignment of chromosomes along the cell's equator happens during the metaphase of mitosis.