An experimental protocol requires that a nutrient deficiency be induced to assess its effects on memory. What is the most appropriate study design to use to address this question?

a. randomized controlled trial

b. epidemiological study

c. cell culture study

d. animal experiment

Animal Experiment

Explanation:

Also known as animal testing. It involves the use Animal models which are non human animals in an experiment to test the effects of a variable on its' behavior or biological system.

In the said experimental protocol, it is required that nutrient deficiency be introduced to access its effect on memory cells. Hence an animal model will be needed for this type of experiment making an animal experiment as the appropriate study design to be used.