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20 May, 01:20

To make an mRNA transcript from a gene, the RNA polymerase needs to 'know' where to start and to stop. What are the signals and proteins involved in initiating and terminating RNA polymerase activity in E. coli?

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  1. 20 May, 04:31
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    In E. coli promoter there are two consensus sequence which is located upstream to from the start site of the transcription. One consensus sequence is present at - 10 and one at - 35.

    These consensus sequences are necessary to be recognized to start the transcription. It is recognized by the sigma factor which is a part of RNA polymerase. After recognition transcription starts.

    The termination of transcription occurs by a rho protein. Rho is ATP dependent RNA stimulated helicase which disrupts the complex of RNA-DNA and terminates transcription.
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