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27 June, 18:36

A protein subunit from an enzyme is part of a research study and needs to be characterized. A total of 0.160 g of this subunit was dissolved in enough water to produce 2.00 ml of solution. At 28 ∘c the osmotic pressure produced by the solution was 0.138 atm. What is the molar mass of the protein?

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  1. 27 June, 21:49
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    The Molar Mass (MM) of the protein is 14318.5g/mole

    Explanation:

    Number of moles (n) of the protein = mass/MM = 0.16/MM

    From the ideal gas equation

    PV = nRT

    P = 0.138 atm, V = 2ml = 2cm^3, n = 0.16/MM mole, R = 82.057 cm^3. atm/gmol. K, T = 28°C = 28+273 = 301K

    0.138 * 2 = 0.16/MM * 82.057 * 301

    MM = 3951.9/0.276 = 14318.5g/mole
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