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30 January, 17:11

What must eukaryotes do to initiate transcription of a gene?

a) Bind RNA Polymerase to displace histone proteins that binding DNA in the promoter region.

b) Recruit general transcription factors to produce an open complex and then recruit RNA Polymerase.

c) Recruit the transcription factors and RNA Polymerase that compose the pre-initiation complex.

d) Open the DNA template and then bind RNA Polymerase at the transcriptional initiation site.

e) Bind transcription factors from enhancer sequences to the RNA Polymerase.

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  1. 30 January, 19:25
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    c) Recruit the transcription factors and RNA Polymerase that compose the pre-initiation complex.

    Explanation:

    Transcription leads to formation of RNA from DNA. The process is carried out by RNA Polymerase which forms a RNA strand according to the template DNA strand.

    It starts by the formation of pre-initiation complex. RNA Polymerase along with transcription factors binds to the promoter region of DNA and forms the pre-initiation complex. Generally the eukaryotic promoters have a sequence called as TATA box where the transcription factors bind first. Eventually RNA polymerase also binds to the region and the DNA strands separate starting the transcription process.
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