During transcription, the template DNA strand is read 5'-to-3' by the RNA polymerase complex.

a. True

b. False

B. False.

Explanation:

Transcription is the process of RNA synthesis. The enzyme RNA polymerase makes the RNA copies of the template DNA strand. RNA polymerase binds to the promoter sequence present at the template DNA strand and reads it in 3' to 5' direction to make the new RNA strand in 5' to 3' direction.

RNA chain is made by joining the ribonucleotides by phosphodiester bonds that are formed when the free 3' end of one ribonucleotide exhibits a nucleophile attack at 5' phosphate of the next ribonucleotide. Therefore, RNA synthesis always occurs in 5' to 3' direction while the template strand is read in 3' to 5' direction.