How would someone measure the effect of pH on the rate

ofenzyme reaction?

It can be determined by measuring product appearance or reagent disappearance also enzymatic activity (described below)

Explanation:

Effect of pH on enzymatic activity

PH effect: affects the state of dissociation of the groups, although all proteins are not affected in the same way because some do not have dissociable groups. Most enzymes have an optimal pH. If there are small changes in pH, the enzyme is not denatured. The pH can affect in two ways:

The substrate binding is better or worse than before.

That affects the catalytic speed of the reaction.

The enzymatic speed is measured in M / t and the enzymatic activity in mol / t, and the international unit μmol / min, amount of enzyme that transforms a micromol from substrate into product in one minute under optimal conditions. Another unit is the enzymatic amount that is required to transform 1 mol / s and is called katal.