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14 July, 16:39

If you start with 5ng of template DNA. How much would you have

after 10 cycles?

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  1. 14 July, 16:52
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    5.12μg of DNA

    Explanation:

    PCR is a method use to amplify DNA. It often require primers (short sequence) nucleotides, heat stable polymerase enzymes, and thermocycler.

    DNA strands are double by every cycle of PCR and it denoted by the formula 2^n where n is the number of cycle.

    starting with 5ng,

    the amount after 10 cycles = 2¹⁰ * 5 ng = 5.12μg of DNA
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