What are the main differences and similarites between the

radioimmunodiffusion (rid) and oucterlony plate technique?

- Similarities both are diffusion process

- wells are involved.

Radial Immunodiffusion (RID) Quantitative test technique quantitative radial immunodiffusion is based upon the reaction between an Ag, and a specific Ab during a diffusion period.

• Ag placed in a well diffuses into an agar containing the Ag (anti-IgG looking for serum IgG).

• The Ag-Ab interaction is manifested by well-defined ring of precipitation around the Ag well.

Explanation:

While Ouchterlony double diffusion (Immunodiffusion) Qualitative test It is based upon the simultaneous application of Ag and Ab in separate but adjacent wells of an agar plate.

• As the materials diffuse toward one another, ppt. lines form resulting from the Ag-Ab interactions.

• If multiple wells of Ag are positioned around an Ab well on the same plate, several patterns of reactivity may be observed.