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23 March, 23:38

During purification of many cytosolic proteins, it is important to

include either dithiothreitol or b-mercaptoethanol in the

buffers. Why?

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Answers (1)
  1. 24 March, 01:31
    0
    To reduce thiol-groups of the protein

    Explanation:

    Dithiothreitol of b-mercaptoethanol act as reducing agents. Including them in a buffer would prevent oxidation of thiol-groups in amino acids of the protein and preventing its denaturation.
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