You count 47 cfu on a spread plate. The plate was prepared by spreading 0.2 ml of a 1:10,000 dilution of the original sample. What is was the concentration of the original culture (in cells/ml) ?

Question

Biology

Amiyah Moon

24 June, 15:24

You count 47 cfu on a spread plate. The plate was prepared by spreading 0.2 ml of a 1:10,000 dilution of the original sample. What is was the concentration of the original culture (in cells/ml) ?

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Answers (1)

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Mills · Answer 1

Answer: 2.35 x 10^6 cfu/ml

Explanation:

Concentration of the original culture:?

Dilution factor: 10^4

Colony count per plate: 47cfu

Volume of cultured plate: 0.2ml

Concentration of the original culture = Colony count per plate x Dilution factor

Volume of cultured plate

Concentration of the original culture = 47 x 10^4

0.2

Concentration of the original culture = 2.35 x 10^6 cfu/ml

You can further take the log of your answer which would be:

Log (2.35 x 10^6) = 6.371