What might be a use for fluorescence in situ hybridization (FISH) ?

a. For identification of a specific gene in a DNA extraction by hybridization to a DNA probe

b. For identification of a specific gene by hybridization to a DNA probe within live cells that have had their DNA denatured by heat.

c. For identification of an mRNA within an RNA extraction by hybridization to a DNA probe.

d. For identification of both mRNA and DNA in cellular extracts using an RNA probe

e. None of the above

Question

Biology

Karson Henson

8 January, 21:09

What might be a use for fluorescence in situ hybridization (FISH) ?

a. For identification of a specific gene in a DNA extraction by hybridization to a DNA probe

b. For identification of a specific gene by hybridization to a DNA probe within live cells that have had their DNA denatured by heat.

c. For identification of an mRNA within an RNA extraction by hybridization to a DNA probe.

d. For identification of both mRNA and DNA in cellular extracts using an RNA probe

e. None of the above

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Answers (1)

Know the Answer?

Draven Edwards · Answer 1

B.

Explanation:

Flourescent insitu hybridization-This technique permits the direct analysis of sequences of DNA or RNA in tissues

-Through this technique various chromosomal abnormalities can be determined as it focuses on that part of the DNA for which a complementary probe has been used.

-The method involves the denaturation of DNA in a tissue section followed by the application of a labeled probe which is complementary to the sequence of interest.