During transcription in eukaryotes, a type of RNA polymerase called RNA polymerase II moves along the template strand of the DNA in the 3'→5' direction. However, for any given gene, either strand of the double-stranded DNA may function as the template strand. For any given gene, what ultimately determines which DNA strand serves as the template strand?

Transcription is defined as formation of messenger RNA (mRNA) from the DNA. Transcription occurs through RNA polymerase.

Explanation:

For transcription one strand serves as template and other strand works as coding strand. Coding strand is identical to the RNA strand forms after transcription using the template strand. Promoter gene base sequence will ultimately determines which DNA strand will serve as template strand for transcription. It also depends on where the genes are present which will be transcribed and ultimately forms the proteins.