You are trying to match DNA found at a crime scene with two possible suspects. Why would it be a better use of your time and resources to analyze the DNA using a double restriction digest rather than a single digest

Answer: A double restriction digest

Explanation: A double restriction digest is a process were two restriction enzymes are used to digest DNA in a single reaction. in double restriction digest you will observe two bands, while in single restriction digest you will observe just one band. In double restriction digest no

Self - ligating plasmid because it create mismatch ends. It also aids in directional in section while single disgestion you have to screen your clones to be sure to get your plasmid with correct insert orientation.