How is a gram-positive cell envelope (cell wall) different than a gram-negative cell envelope

Gram Positive Gram Negative

Retain the crystal violet stain

(primary stain) and appear in purple

under microscope. Does not retain the crystal violet

stain, appear as pink with safranin

(counter stain)

2. The cell wall primarily composed

of peptidoglycan Cell wall contain three main

components - (1) Lipopolysaccharides,

(2) Lipoproteins and (3) Peptidoglycan

3. Thick and

homogenous cell wall. Thin and heterogeneous cell wall.

4. Less lipid content Comparatively more lipid content

5. Teichoic acid present Teichoic acid absent

6. LPS absent LPS present