g As a second step in thenerd-proposal, Wouldbe Fiancé needs to obtain DNA fragments that are the right size to make up the letters spelling out "Will you marry me?" How would they do that? A) Use restriction enzymes to cut the DNA at specific sequence sites. B) Waitfor helicase to unzip the genes. C) Use DNA ligase to stick together the Okazaki fragments. D) Alter the reading frame of the mRNA.

Question

Biology

Isabel Barton

25 August, 20:00

g As a second step in thenerd-proposal, Wouldbe Fiancé needs to obtain DNA fragments that are the right size to make up the letters spelling out "Will you marry me?" How would they do that? A) Use restriction enzymes to cut the DNA at specific sequence sites. B) Waitfor helicase to unzip the genes. C) Use DNA ligase to stick together the Okazaki fragments. D) Alter the reading frame of the mRNA.

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Answers (1)

Know the Answer?

Susan Roberson · Answer 1

A) Use restriction enzymes to cut the DNA at specific sequence sites.

Explanation:

Restriction enzymes cut DNA in specific sites. There are high and low frequency endonucleases. The easiest to get fragments to make the words: WILL YOU MARRY ME? is to amplify PCR fragments of known size and sequence, therefore, use restriction enzymes to cut the fragments of the desired size. It will be very useful make an in silico digestion to predict all the fragment sizes to be obtained, and a simulation of the gel before loading it.