Which of the following statements about Polymerase Chain Reactions is wrong?

(A) DNA is briefly heated to break the hydrogen bonds holding two strands together.

(B) Primers hybridize to sequences at opposite ends of the target sequence on the same strand.

(C) DNA is cooled to allow primers to form hydrogen bonds with the ends of the target sequence.

(D) The three steps of PCR are denaturation, annealing, and extension.

(E) PCR produces specific DNA fragments for cloning.

(F) Taq polymerase adds nucleotides to the 3' end of each primer.

The correct answers will be option-B

Explanation:

The polymerase chain reaction is the technique which involves the chain of reaction. The reactions are performed which polymerises or make exact copies of the DNA in higher number from a small sample in vitro-conditions.

The PCR technique is based on the DNA replication mechanism which involves the DNA primers and Taq polymerase, a type of DNA polymerase enzyme.

The PCR proceeds in three steps that are denaturation, annealing, and extension in which the DNA is denatured to break the hydrogen bonds, the addition of the primers at both ends called reverse and forward primers which function simultaneously, synthesis of DNA and extension in which the sample is cooled.

Since the primers are added to both the strands and not on the same strands therefore, the selected option is the correct answer.