What will occur if solution A containing 400 mosmol/L non-penetrating solute is separated by a biological membrane from solution B containing 600 mosmol/L non-penetrating solute?

The volume of solution B will increase

Explanation:

In a non-penetrating solute, there is denial of a solute to cross the cell membrane. This implies that the solute will not be able to cross the cell membrane. So if the biological membrane is serving as a partition between a solution A containing 400 mosmol/L non-penetrating solute and a solution B containing 600 mosmol/L non-penetrating solute, The volume of solution B will increase.