All of the following involve molecular biology techniques except ligating DNA fragments together. using a DNA polymerase to copy DNA via PCR. creation of Okazaki fragments. using restriction enzymes to cut specific sequences of DNA.

creation of Okazaki fragments.

Explanation:

Replication of the lagging strand of DNA is discontinuous due to the antiparallel polarity of the two strands of DNA and the ability of DNA polymerase to replicate DNA in 5' to 3' direction only. The problem of synthesis of DNA in 5' to 3' direction on both template strands is solved by the synthesis of the short DNA segments at a small distance on one of the template DNA strands. These small fragments that are formed are called Okazaki Fragments and joined together by DNA ligase to form the lagging strands in DNA replication. Each of the Okazaki fragment is 100-1000 nucleotides long.

It is a natural process that occurs during DNA replication and therefore, does not include any molecular technique.

The correct answer is-creation of Okazaki fragments

Explanation:

Molecular technique is the methods that are used in molecular biology and are also common in other life science streams like biochemistry, biophysics, genetics and involves the manipulation of genes or DNA and their analysis.

Okazaki fragments are formed during the DNA replication in a discontinuous way so many fragments of DNA are formed which are called Okazaki fragments then these fragments are joined by ligase enzymes.

So Okazaki fragments are made autonomously during replication therefore no specific molecular technique is required to create Okazaki fragments.