You perform a ligation with NcoI/NotI-digested pET-41a vector and the egfp insert from pEGFP-N1 (as in the lab). You then transform the ligation mix into E. coli and plate on LB medium containing kanamycin. Consider the following unexpected outcomes, and suggest controls: a. Nothing grows. What controls might you design to determine whether the E. coli cells are viable (alive) versus whether the E. coli is competent
+2
Answers (1)
Know the Answer?
Not Sure About the Answer?
Find an answer to your question ✅ “You perform a ligation with NcoI/NotI-digested pET-41a vector and the egfp insert from pEGFP-N1 (as in the lab). You then transform the ...” in 📘 Biology if you're in doubt about the correctness of the answers or there's no answer, then try to use the smart search and find answers to the similar questions.
Home » Biology » You perform a ligation with NcoI/NotI-digested pET-41a vector and the egfp insert from pEGFP-N1 (as in the lab). You then transform the ligation mix into E. coli and plate on LB medium containing kanamycin.