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21 May, 03:52

After you performed the freeze-thaw cycle to lyse the bacteria, you pelleted the debris using a centrifuge. What two reasons did this pellet fluoresce green? (Yes, I know it contained GFP ... but why?)

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  1. 21 May, 07:14
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    Green Fluorescent Protein Labelled Bacteria

    Explanation:

    Green Fluorescent Protein labeling is very useful in studying prokaryotes. It is highly likely that the bacteria was also labelled. That's why after lysis and density level differentiation in a centrifuge the pellet start glowing green.
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