Which suspect's DNA matches that found at the crime scene? Does this automatically mean that the suspect is guilty? 2. What possible experimental problems could occur to invalidate the results? 3. If only Restriction Enzyme 1 was used, would the interpretation be the same?

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Biology

Demarcus Barnes

11 January, 16:14

Which suspect's DNA matches that found at the crime scene? Does this automatically mean that the suspect is guilty? 2. What possible experimental problems could occur to invalidate the results? 3. If only Restriction Enzyme 1 was used, would the interpretation be the same?

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Jovanny Boyle · Answer 1

RFLP analysis of genomic DNA is facilitated by Southern blot analysis. After electro-phoresis, DNA fragments in the gel are denatured by soaking in an alkali solution. This causes double-stranded fragments to be converted into single-stranded form (no longer base-paired in a double helix). A replica of the electrophoretic pattern of DNA fragments in the gel is made by transferring (blotting) them to a sheet of nitrocellulose or nylon membrane. This is done by placing the membrane on the gel after electro-phoresis and transferring DNA fragments to the membrane by capillary action or electro-transfer. DNA, which is not visible, becomes permanently adsorbed to the membrane, that can then be manipulated easier than gels.