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18 June, 00:20

Identify the buffer solution that can be used for eluting a transcription factor bound to a DNA affinity column. a. high sugar concentration b. high imidazole concentration c. a mixture of free DNA nucleotides d. high salt concentration

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  1. 18 June, 00:55
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    Option D is correct

    Explanation:

    Gradient elution of transcription factor is facilitated by disrupting DNA - transcription factor interaction by increasing concentration of salt in other to increase the ionic strength.
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