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23 July, 15:51

What happens if you attempted to perform gel electrophoresis on the sample from the agar electrophoresis? (without doing PCR)

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  1. 23 July, 18:49
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    Gel electrophoresis can be described as a technique which is usually used in labs to separate DNA molecules on the basis of their size and charge.

    If PCR is not performed before the gel electrophoresis, this means that the concentration will be very low. When the DNA will be uncut, it will be too large and will stop at the start of the gel. Hence, in such type of DNA the bands would not run due to the low concentration of DNA. PCR is the technique which us used to amplify a DNA. It comprises of the steps of denaturing the DNA strands, adding nucleotides for making the new strands, and then extending the strand. PCR will allow million of copies of the DNA to be made. Hence, PCR makes the DNA of the size fragment that we need for gel electrophoresis to occur.
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