A researcher is using the pUC18 vector to introduce a human gene into bacteria. He exposes bacteria to this recombinant molecule and then grows these organisms in culture. He is very surprised to find that all of the resulting bacterial colonies are blue and survive treatment with antibiotics. What can he conclude from these results?

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Biology

Nicholas Stanton

13 April, 07:55

A researcher is using the pUC18 vector to introduce a human gene into bacteria. He exposes bacteria to this recombinant molecule and then grows these organisms in culture. He is very surprised to find that all of the resulting bacterial colonies are blue and survive treatment with antibiotics. What can he conclude from these results?

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Freddy · Answer 1

The results from this experiment conclude that all of the resulting bacteria contain the pUC18 plasmid without the human gene.

The method of genetic engineering might seem very easy but in actual it is a very difficult task and most of the time a person won; t get the expected results. The chances of a vector to actually take up a recombinant DNA are very less. Even after this, the chances of a recombinant plasmid to enter a bacterial cell by transformation are very few.