What is the indicator we are using in this titration? In your own words, what is the purpose of grinding the spirulina? Should the ground silica and spirulina mixture be suspended in deionized water, exhibit water, or buffer? Why? What is the color of the folded phytobilliprotein with the bound cofactor? How do you expect it to change as you add acid or base?

What is the indicator we are using in this titration?

a deep blue indicator

Explanation:

What is the indicator we are using in this titration?

a deep blue indicator

the purpose of grinding the spirulina is to be able to break the cell into smaller particles that can dissolve well in a solution.

when powder spirulina is mixed with silica the cells are easily break even.

Should the ground silica and spirulina mixture be suspended in deionized water, exhibit water, or buffer? Why?

ground silica and spirulina mixture be suspended in deionized water because its dissolved and mixed well in water than any other solvent.

What is the color of the folded phytobilliprotein with the bound cofactor?

A blue color is observed when phytobilliprotein is folded.

How do you expect it to change as you add acid or base?

when a base solvent is use for the solution of silica and spirulina (e. g of base NaOH) is used, the colour obseved will be pale yellow or green color.

when a acidic solvent is use for the solution of silica and spirulina (e. g of base HCL) is used, the colour obseved will be colorless solution.

When a base and acidic solvent is used, it causes a changed in the charge state of the protein and changes the interaction thereby causing unfolding.