In which direction does DNA replication take place?

5'-3'

3'-5'

5'

3'

In the 5'-3' direction

Explanation:

DNA is made up of nucleotides which are derived from phosphate, nucleobase and a deoxyribose, which is a sugar molecule. Addition of a new nucleotide requires a free hydroxyl group (-OH) of the sugar molecule. This is usually at 3' end of the DNA strand. Thus, the DNA polymerase reads the strand from the 5'-3' end adding a new nucleotide at the 3' end.