Ask Question
26 May, 20:54

In blue-white screening, what do blue colonies represent?

cells that have not taken up the plasmid vector

cells with recombinant plasmids containing a new insert

cells containing empty plasmid vectors

cells with a non-functional lacZ gene

+2
Answers (1)
  1. 26 May, 22:02
    0
    The correct option is : cells containing empty plasmid vectors

    Explanation:

    The blue-white screen is a technique which involves the rapid identification of the recombinant bacteria in a vector-based molecular cloning experiment. In this method, a DNA ligated vector is inserted in a host cell which is viable for transformation and grown in presence of X-gal.

    The cells that are transformed with the vectors having the recombinant DNA produce the white colonies. Whereas, the cells transformed with empty vector i. e. the non-recombinant plasmids, produce the blue colonies.
Know the Answer?
Not Sure About the Answer?
Find an answer to your question ✅ “In blue-white screening, what do blue colonies represent? cells that have not taken up the plasmid vector cells with recombinant plasmids ...” in 📘 Biology if you're in doubt about the correctness of the answers or there's no answer, then try to use the smart search and find answers to the similar questions.
Search for Other Answers