In these protein assays, the absorbance is plotted on the y-axis, while concentration is plotted on the x. If the equation of the line for your standard curve is y = 0.057x + 0.069 with an R^2 value of 0.9913, and your sample has an absorbance of 1.111, what is the concentration of your sample?

18.28

Explanation:

Given:

Relation between the absorbance (y) and the concentration (x) as;

y = 0.057x + 0.069

now,

we have to calculate concentration (x) for the given value of absorbance (y) = 1.111

thus, on substituting the value of y = 1.111 in the above relation, we get

1.111 = 0.057x + 0.069

or

x = 18.28

hence, the value of concentration of sample is 18.28