What factors influence the amount of DNA you have?

The factors are: The size of the sample, the extraction method, the method of purification and preservation of the DNA.

The quantity and quality of DNA can be greatly improved by performing PCR.

* Purification of nucleic acids is a key step for functional studies in biology. Because of the great diversity of living organisms in nature, purification becomes a real challenge for researchers, especially cell lysis.

The evolution of lysis methods has made it possible to improve efficiency, speed and simplicity. But, this lysis step remains a crucial step for the efficiency and the quality of the purification.