Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as b-lactamase), an enzyme present in some penicillin - resistant bacteria. the mass of this enzyme in staphylococcus aureus is 29.6 kd. the amount of penicillin hydrolyzed in 1 minute in a 10-ml solution containing 1029 g of purified penicillinase was measured as a function of the concentration of penicillin. assume that the concentration of penicillin does not change appreciably during the assay.

Question

Biology

Kyra

15 May, 18:27

Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as b-lactamase), an enzyme present in some penicillin - resistant bacteria. the mass of this enzyme in staphylococcus aureus is 29.6 kd. the amount of penicillin hydrolyzed in 1 minute in a 10-ml solution containing 1029 g of purified penicillinase was measured as a function of the concentration of penicillin. assume that the concentration of penicillin does not change appreciably during the assay.

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Answers (2)

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Ella Beasley · Answer 1

The answer is 5.22 mM.

Explanation:

The calculation of Vmax and Km is as follows:

y = 7.6225x + 1.4602

so, when x = 0

x = 1 : Vmax = 1.4602.

Vmax = 1 : 1.4602 = 0.68.

Thus, Vmax of the enzyme is 0.68.

The calculation of (km) is as follows:

Y = 7.6225X + 1.4602

so, when y = 0

x = 1 : Km = 5.22.

Billie · Answer 2

5.22 mM.

Explanation:

using y=mx+c equation

y = 7.6225x + 1.4602

so, when x = 0

x = 1 : Vmax = 1.4602.

Vmax = 1 : 1.4602 = 0.68.

Thus, Vmax is 0.68.

Y = 7.6225X + 1.4602

so, when y = 0

x = 1 : Km = 5.22.