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17 December, 12:57

The chemical stockroom also contains a solution labeled unknown. This solution contains a 0.10M solution of a "synthetic base". Synthetic bases are designed and used by biologists to manipulate DNA. Which base, A, C, T or G, is this unknown synthetic base most like, in terms of its binding characteristics?

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  1. 17 December, 15:59
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    AT indicates A and T bound together, AC indicates A and C bound together, etc.

    Explanation:

    In the '30s and '40s, biologists knew that the agent for heredity was in thread-like chromosomes, made of the molecule DNA. They also knew that DNA was made of four bases, (A) denine, (G) uanine, (C) ytosine, and (T) hymine. The race was on to be first to determine how they fit together, and how they carried hereditary information.

    DNA

    In 1953, James Watson and Francis Crick unraveled the mystery, by showing that DNA had the double-helix structure shown to the left. The information is stored as the sequence of bases: G, C, A, and T, on the strand of DNA running up one side of the double helix. On the opposing strand, the sequence is mirrored such that a C is paired with every G, and an A is paired with every T. This pairing is essential to the ability of DNA to replicate and to send its information out into the cell to direct protein synthesis.

    You will be provided with stock solutions of all four bases. These are labeled dAMP for A, dCMP for C, dGMP for G, and dTMP for T to reflect the fact that these bases are only soluble as single bases in their d-MonoPhosphate form. Each of these solutions has a concentration of 0.10M. In the following, feel free to combine these solutions in any way you see fit. Note that the "Solution Info" window shows the concentrations of all chemical species in the currently-selected container. In this window, AT indicates A and T bound together, AC indicates A and C bound together, etc.
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