If we have a plate that has 2500 colonies on it, can we use this plate to calculate CFU's

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Biology

Suzan

16 March, 11:28

If we have a plate that has 2500 colonies on it, can we use this plate to calculate CFU's

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Backbone · Answer 1

No, the solution with bacteria in it needs to be diluted in order to be in the range of 30 - 300.

A colony-forming unit - CFU is used to estimate the number of viable (ability to divide by binary fission) microbes in a sample.

A sample of microbes is diluted and spread on a plate. Then, the number of colonies is counted, assuming that each colony have grown from a single colony-forming unit, or CFU.

Plates with more than 300 colonies are difficult to count while those with less than 30 colonies give statistically unreliable numbers of colonies to count.