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3 September, 07:35

What are free DNA nucleotides needed for in PCR?

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  1. 3 September, 10:05
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    Free nucleotides are required in the PCR for the elongation for the new DNA strand during replication by the polymerase. Remember that during DNA replication, a primer is recruited at the initiation site then DNA polymerase enzyme elongates the primer by adding nucleotides (dNTPs) at the 3' end of the primer and catalyzing a phosphodiester bond.
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