When the stains are eluted crystal violet is retained near the top of the soil column, whereas eosin y is retained throughout the length of the column?

Crystal Violet retained towards the top of the soil column while Eosin Y was seen throughout the length of column because they have different charges. Cation exchange and polarity, have much to do with each dye, and each dye went to different places. This process depends on binding sites and the charges of the soil, which is also related to water-holding capacities. Since Crystal Violet was eager to accept a hydrogen molecule and our soil had a negative charge, so Crystal Violet remained towards the top and leave the site positively charged. Polar Eosin Y has a negative net charge, like our soil, and diffused more rapidly through the length of the soil column.

Answer:The soil samples have more negative charged ion-binding sites per volume that positively charged ion-binding sites.

Explanation:The soil samples have more negative charged ion-binding sites per volume that positively charged ion-binding sites. Therefore, a small volume of soil easily retains all the Crystal Violet but a large volume is needed to retain the eosin y