Ask Question
18 August, 02:18

A mixture of the amino acids leucine, glutamate, and arginine, is added to a cation exchange column at neutral ph. in what order will they elute from a cation exchange column

+3
Answers (1)
  1. 18 August, 03:13
    0
    Cation-exchange chromatography is used when the molecule of interest is positively charged, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. So, the amino acids with negative charge will elute the first. Glutamate, leucin, arginine is the order of elution because of their pI values ~3, ~6 ~10.
Know the Answer?
Not Sure About the Answer?
Find an answer to your question ✅ “A mixture of the amino acids leucine, glutamate, and arginine, is added to a cation exchange column at neutral ph. in what order will they ...” in 📘 Biology if you're in doubt about the correctness of the answers or there's no answer, then try to use the smart search and find answers to the similar questions.
Search for Other Answers