To produce transgenic bacteria that make insulin, which of the steps listed below would a scientist do last?

A. Insert the human insulin gene into a plasmid.

B. Extract the insulin from the bacterial culture.

C. Cut out the insulin gene from human DNA.

D. Transform bacteria with the recombinant plasmid.

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The last thing that the scientist would do in producing transgenic bacteria for generating insulin would be to extract the insulin from the bacterial culture. The proper sequence of the steps to be done by the scientist should be:

Cut out the insulin gene from human DNA.

Insert the human insulin gene into a plasmid

Transform bacteria with the recombinant plasmid.

Extract the insulin from the bacterial culture.